CHI3L1 ELISA Kit (Rat) (OKCD02509)

Catalog No: OKCD02509

Size:96WELLS

Rat CHI3L1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-98%	80-89%	89-101%	91-98%
  EDTA Plasma (n=5)	97-105%	78-95%	94-103%	89-98%
  Heparin Plasma (n=5)	79-97%	99-105%	87-95%	92-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase-3-like Protein 1 (CHI3L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase-3-like Protein 1 (CHI3L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase-3-like Protein 1 (CHI3L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chitinase-3-like Protein 1 (CHI3L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-103	94
  EDTA Plasma (n=5)	78-95	83
  Heparin Plasma(n=5)	81-105	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chitinase-3-like Protein 1 (CHI3L1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chitinase-3-like Protein 1 (CHI3L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CHI3L1 Microplate: 96 Wells (12 x 8 Well strips)
  • CHI3L1 Lyophilized Standard: 2
  • 100X Biotinylated CHI3L1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.28 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycoprotein 39, Cartilage (GP39). No significant cross-reactivity or interference between Glycoprotein 39, Cartilage (GP39) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Chi3l1
• Gene Full Name: chitinase 3 like 1
• Alias Symbols: cartilage glycoprotein 39;CGP-39;Chil1;chitinase 3-like 1 (cartilage glycoprotein-39);chitinase 3-like 1 protein;chitinase-3-like protein 1;GP-39.
• NCBI Gene Id: 89824
• Protein Name: Chitinase-3-like protein 1
• Description of Target: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung (By similarity).
• Uniprot ID: Q9WTV1
• Protein Accession #: NP_001296749.1
• Nucleotide Accession #: NM_001309820.1
• Protein Size (# AA): 381