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Catalog No: OKCD00887 (Formerly GWB-KBBQS4)
Size:96WELLS
Price: $700.00
SKU
OKCD00887
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Datasheets/ManualsPrintable datasheet for CHGB ELISA Kit (Mouse) (OKCD00887)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-97%89-103%88-103%86-101%
EDTA plasma(n=5)80-101%90-98%81-96%99-105%
heparin plasma(n=5)94-105%79-89%80-89%93-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin B (CHGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromogranin B (CHGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromogranin B (CHGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromogranin B (CHGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)83-10187
EDTA plasma(n=5)82-9286
heparin plasma(n=5)89-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromogranin B (CHGB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromogranin B (CHGB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Chgb Microplate96 Wells (12 x 8 Well Strips)
Chgb Lyophilized Standard2 x 20 ng
100X Biotinylated Chgb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.118 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Chromogranin B (CHGB). No significant cross-reactivity or interference between Chromogranin B (CHGB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolChgb
Gene Full Namechromogranin B
Alias SymbolscgB;Chromogranin-B;Scg-;Scg-1;secretogranin I;secretogranin-1;sgI.
NCBI Gene Id12653
Protein NameSecretogranin-1
Description of TargetSecretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Uniprot IDP16014
Protein Accession #NP_031720
Nucleotide Accession #NM_007694.4
Protein Size (# AA)677
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