Catalog No: OKEH03318
Size:96T
Price: $525.00
SKU
OKEH03318
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CHGA ELISA Kit (Rat) (OKEH03318) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Chga has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Chga is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Chga captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-Chga Microplate96 Wells (12 x 8 Well strips)
Chga Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Chga Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.091 ng/mL
SpecificityNatural and recombinant Rat Chromogranin-A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCHGA
Gene Full Namechromogranin A
Alias SymbolsCGA
NCBI Gene Id1113
Protein NameP10354
Description of TargetChromogranin-A
Uniprot IDhttps://www.uniprot.org/uniprot/P10354
Protein Accession #NP_001266.1
Nucleotide Accession #NM_001275.3
Protein Size (# AA)457
Molecular Weight51 kDa
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