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Datasheets/Manuals | Printable datasheet for CHEK2 Antibody (Phospho-Thr383) (OAAF00144) |
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Predicted Species Reactivity | Human|Monkey|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr383. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Specificity | Chk2 (Phospho-Thr383) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 383. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:20000 |
Reference | Shannon L. Gibson, Cancer Res., Dec 2005; 65: 10734 - 10741. Jinwoo Ahn, J. Biol. Chem., Dec 2002; 277: 48418 - 48426. Nabil H. Chehab, Genes & Dev., Feb 2000; 14: 278. |
Gene Symbol | CHEK2 |
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Gene Full Name | checkpoint kinase 2 |
Alias Symbols | CDS1;cds1 homolog;Checkpoint kinase 2;checkpoint-like protein CHK2;CHK2;CHK2 checkpoint homolog;hCds1;HuCds1;LFS2;PP1425;RAD53;serine/threonine-protein kinase Chk2. |
NCBI Gene Id | 11200 |
Protein Name | Serine/threonine-protein kinase Chk2 |
Description of Target | Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978). |
Uniprot ID | O96017 |
Protein Accession # | NP_001005735.1 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse|Rat".
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How long will it take to receive "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
This target may also be called "CDS1;cds1 homolog;Checkpoint kinase 2;checkpoint-like protein CHK2;CHK2;CHK2 checkpoint homolog;hCds1;HuCds1;LFS2;PP1425;RAD53;serine/threonine-protein kinase Chk2." in publications.
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What is the shipping cost for "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "60 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CHEK2 Antibody (Phospho-Thr383) (OAAF00144)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CHEK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CHEK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CHEK2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CHEK2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CHEK2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CHEK2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.