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Catalog No: ARP30335_P050
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ARP30335_P050
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Datasheets/ManualsPrintable datasheet for anti-CHEK2 (ARP30335_P050) antibody
Product Info
Tested Species ReactivityHuman
Predicted Species ReactivityHuman
Product FormatLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ClonalityPolyclonal
HostRabbit
ApplicationWB
Reconstitution and StorageFor short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
ImmunogenThe immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2
PurificationAffinity Purified
Predicted Homology Based on Immunogen SequenceHuman: 100%
Peptide SequenceSynthetic peptide located within the following region: SRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQ
Concentration0.5 mg/ml
Blocking PeptideFor anti-CHEK2 (ARP30335_P050) antibody is Catalog # AAP30335 (Previous Catalog # AAPP00716)
ReferenceLi,H., (2008) Virology 375 (1), 13-23
Gene SymbolCHEK2
Gene Full NameCheckpoint kinase 2
Alias SymbolsCDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425
NCBI Gene Id11200
Protein NameSerine/threonine-protein kinase Chk2
Description of TargetIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Uniprot IDB7ZBF3
Protein Accession #NP_001005735
Nucleotide Accession #NM_001005735
Protein Size (# AA)586
Molecular Weight65kDa
Protein InteractionsSRPK1; PPP2CA; PLK1; CHEK2; PRKDC; ATM; POLR2L; RCHY1; VHL; UBC; NR4A1; ELAVL1; CDC25A; ARHGAP1; PER3; CDC25C; CUL1; CUL4A; KPNA2; APP; STRAP; TP53; BRCA1; RB1; DAPK3; UBR5; PML; MDM2; BRCA2; KAT2B; LATS2; PSME3; TRIM28; RNF8; STAT1; PPP2R5E; PPP2R5D; PPP
  1. What is the species homology for "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".

  3. What buffer format is "CHEK2 Antibody - N-terminal region (ARP30335_P050)" provided in?

    This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    This target may also be called "CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425" in publications.

  5. What is the shipping cost for "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "65kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "CHEK2 Antibody - N-terminal region (ARP30335_P050)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CHEK2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CHEK2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CHEK2"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CHEK2"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CHEK2"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CHEK2"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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