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Catalog No: OKEH04858
Size:96T
Price: $525.00
SKU
OKEH04858
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Chat ELISA Kit (Rat) (OKEH04858) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Chat has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Chat is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Chat captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Chat Microplate96 Wells (12 x 8 Well Strips)
Chat Standard2
Sample Diluent1 x 20 mL
Chat Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.182 ng/mL
SpecificityNatural and recombinant Rat Choline O-acetyltransferase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCHAT
Gene Full NameCholine O-acetyltransferase
NCBI Gene Id290567
Protein NameP32738
Description of TargetCholine O-acetyltransferase
Uniprot IDhttps://www.uniprot.org/uniprot/P32738
Protein Accession #NP_001164064.1
Nucleotide Accession #NM_001170593.1
Protein Size (# AA)640
Molecular Weight72 kDa
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