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Catalog No: OKEH02546
Size:96T
Price: $600.00
SKU
OKEH02546
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology cGMP ELISA Kit (OKEH02546) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-cGMP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated cGMP and incubated. The cGMP found in the sample or standards competes with the biotinylated cGMP for limited binding sites on the immobilized anti-cGMP antibody. Excess unbound biotinylated cGMP and sample or standard cGMP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated cGMP captured in the well and inversely proportional to the amount of cGMP which was contained in the sample or standard.
ELISA Kit Range15.6-1000 pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-cGMP Microplate96 Wells (12 x 8 Well strips)
cGMP Lyophilized Standard2 x 20 ng
Sample Diluent1 x 20 mL
100X cGMP-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: cGMP
Target Name: Cyclic guanosine monophosphate
::Pubchem: 24316
::Chemical formula: C10H12N5O7P
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity5.8 pg/mL
SpecificityNatural and recombinant General Cyclic guanosine monophosphate
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolscGMP, Cyclic guanosine monophosphate, cGMP
Protein NamecGMP
Description of TargetCyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from guanosine triphosphate (GTP). cGMP acts as a second messenger much like cyclic AMP. Its most likely mechanism of action is activation of intracellular protein kinases in response to the binding of membrane-impermeable peptide hormones to the external cell surface. Guanylate cyclase (GC) catalyzes cGMP synthesis. This enzyme converts GTP to cGMP. In turn, peptide hormones such as the atrial natriuretic factor activate membrane-bound GC, while soluble GC is typically activated by nitric oxide to stimulate cGMP synthesis.
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