CGB ELISA Kit (Human) : 96 Wells (OKEH00451)

Catalog No: OKEH00451

Size:96T

Human CGB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CGB ELISA Kit (Human) (OKEH00451) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CGB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human CGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CGB captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.9% (n = 20)
  Mean Inter-assay CV%: < 7.3% (n = 20)
• ELISA Kit Component:
  • Anti-CGB Microplate: 96 Wells (12 x 8 Well strips)
  • CGB Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CGB: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.35 ng/mL
• Specificity: Natural and recombinant Human Choriogonadotropin subunit beta
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CGB3, CGB5, CGB8
• Gene Full Name: chorionic gonadotropin subunit beta 3, chorionic gonadotropin subunit beta 5, chorionic gonadotropin subunit beta 8
• Alias Symbols: CGB;CGB5;CGB7;CGB8;CG-beta;Choriogonadotropin subunit beta;choriogonadotropin subunit beta 3;chorionic gonadotrophin chain beta;chorionic gonadotropin beta 3 subunit;chorionic gonadotropin beta 5 subunit;chorionic gonadotropin beta 8 subunit;chorionic gonadotropin beta chain;chorionic gonadotropin beta subunit 3;chorionic gonadotropin beta subunit 5;chorionic gonadotropin beta subunit 8;chorionic gonadotropin chain beta;chorionic gonadotropin, beta polypeptide;chorionic gonadotropin, beta polypeptide 5;chorionic gonadotropin, beta polypeptide 8;HCG;hCGB;LHB;luteinizing hormone beta subunit.
• NCBI Gene Id: 1082, 93659, 94115
• Protein Name: P0DN86
• Description of Target: Choriogonadotropin subunit beta 3
• Uniprot ID: https://www.uniprot.org/uniprot/P0DN86
• Protein Accession #: NP_000728
• Nucleotide Accession #: NM_000737
• Protein Size (# AA): 165
• Molecular Weight: 17 kDa