Catalog No: OKEH00450
Size:96T
Price: $325.00
SKU
OKEH00450
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CGA ELISA Kit (Rat) : 96 Wells (OKEH00450)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CGA ELISA Kit (Rat) (OKEH00450) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat CGA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat CGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat CGA captured in well.
ELISA Kit Range0.78-50mIU/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CGA Microplate96 Wells (12 x 8 Well strips)
CGA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CGA-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.39mIU/mL
SpecificityNatural and recombinant Rat Glycoprotein hormones alpha chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCga
Gene Full Nameglycoprotein hormones, alpha polypeptide
Alias Symbolsanterior pituitary glycoprotein hormones common subunit alpha;follicle-stimulating hormone alpha chain;follitropin alpha chain;FSH-alpha;glycoprotein hormone alpha subunit;glycoprotein hormones alpha chain;glycoprotein hormones, alpha subunit;Gpa1;LSH-alpha;luteinizing hormone alpha chain;lutropin alpha chain;pituitary hormone alpha subunit;thyroid-stimulating hormone alpha chain;thyrotropin alpha chain;TSH-alpha.
NCBI Gene Id116700
Protein NameP11962
Description of TargetGlycoprotein hormones alpha chain
Uniprot IDhttps://www.uniprot.org/uniprot/P11962
Protein Accession #NP_446370.2
Nucleotide Accession #NM_053918.2
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