Catalog No: OKEH00689
Size:96T
Price: $525.00
SKU
OKEH00689
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CFI ELISA Kit (Human) : 96 Wells (OKEH00689)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFI ELISA Kit (Human) (OKEH00689) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFI has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFI captured in well.
ELISA Kit Range1.56-100ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CFI Microplate96 Wells (12 x 8 Well strips)
CFI Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CFI Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Complement factor I
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFI
Gene Full Namecomplement factor I
Alias SymbolsAHUS3;ARMD13;C3B/C4B inactivator;C3b-INA;C3BINA;C3b-inactivator;complement component I;complement control protein factor I;complement factor I;complement factor I heavy chain;FI;IF;KAF;Konglutinogen-activating factor;light chain of factor I.
NCBI Gene Id3426
Protein NameP05156
Description of TargetComplement factor I
Uniprot IDhttps://www.uniprot.org/uniprot/P05156
Protein Accession #NP_000195.2
Nucleotide Accession #NM_000204.4
Protein Size (# AA)583
Molecular Weight65 kDa
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