Catalog No: OKEH02689
Size:96T
Price: $425.00
SKU
OKEH02689
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CFD ELISA Kit (Human) : 96 Wells (OKEH02689)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFD ELISA Kit (Human) (OKEH02689) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CFD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFD captured in well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CFD Microplate96 Wells (12 x 8 Well strips)
CFD Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CFD Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity3.9 pg/mL
SpecificityNatural and recombinant Human Complement factor D
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFD
Gene Full Namecomplement factor D
Alias SymbolsADIPSIN;ADN;C3 convertase activator;complement factor D;complement factor D preproprotein;D component of complement (adipsin);DF;PFD;properdin factor D.
NCBI Gene Id1675
Protein NameP00746
Description of TargetComplement factor D
Uniprot IDhttps://www.uniprot.org/uniprot/P00746
Protein Accession #NP_001304264.1
Nucleotide Accession #NM_001317335.1
Protein Size (# AA)253
Molecular Weight27 kDa
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