CETP ELISA Kit (Rabbit) (OKCD00779)

Catalog No: OKCD00779

Size:96WELLS

Rabbit CETP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-103%	82-101%	84-103%	82-90%
  EDTA plasma(n=5)	90-104%	87-101%	92-101%	90-98%
  heparin plasma(n=5)	96-103%	78-89%	96-105%	86-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesteryl Ester Transfer Protein (CETP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesteryl Ester Transfer Protein (CETP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesteryl Ester Transfer Protein (CETP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cholesteryl Ester Transfer Protein (CETP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-101	97
  EDTA plasma(n=5)	96-104	101
  heparin plasma(n=5)	81-90	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CETP Microplate: 96 Wells (12 x 8 Well Strips)
  • CETP Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated CETP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 20.53 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholesteryl Ester Transfer Protein (CETP). No significant cross-reactivity or interference between Cholesteryl Ester Transfer Protein (CETP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CETP
• Alias Symbols: Lipid transfer protein I.
• NCBI Gene Id: 100327267
• Protein Name: Cholesteryl ester transfer protein
• Description of Target: Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed:24293641). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination (By similarity).
• Uniprot ID: P22687
• Protein Accession #: XP_017197724
• Nucleotide Accession #: XM_017342235.1
• Protein Size (# AA): 497