Catalog No: OKEH03715
Size:96T
Price: $625.00
SKU
OKEH03715
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CES5A ELISA Kit (Rat) (OKEH03715) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ces5a antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ces5a and incubated. The Ces5a found in the sample or standards competes with the biotinylated Ces5a for limited binding sites on the immobilized anti-human Ces5a antibody. Excess unbound biotinylated Ces5a and sample or standard Ces5a is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ces5a captured in the well and inversely proportional to the amount of Ces5a which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Anti-Ces5a Microplate96 Wells (12 x 8 Well strips)
Ces5a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Ces5a-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.161 ng/mL
SpecificityNatural and recombinant Rat Carboxylesterase 5A
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCes5a
Gene Full Namecarboxylesterase 5A
Alias Symbolscarboxylesterase 5A;carboxylesterase 615;carboxylesterase 7;carboxylesterase-like urinary excreted protein homolog;cauxin;Ces5;Ces7;epididymis-specific gene 615 protein.
NCBI Gene Id307660
Protein NameQ5GRG2
Description of TargetCarboxylesterase 5A
Uniprot IDhttps://www.uniprot.org/uniprot/Q5GRG2
Protein Accession #NP_001012056.1
Nucleotide Accession #NM_001012056.1
Protein Size (# AA)575
Molecular Weight64 kDa
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