CENPB Antibody - C-terminal region (ARP51969_P050)

Catalog No: ARP51969_P050

WB Suggested Anti-CENPB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:500
Positive Control: Jurkat cell lysate

Host: Rabbit
Target Name: CENPB
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 0.5ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Sheep, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CENPB
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 83%; Horse: 86%; Human: 100%; Mouse: 93%; Pig: 86%; Rat: 100%; Sheep: 86%; Yeast: 100%; Zebrafish: 75%
• Peptide Sequence: Synthetic peptide located within the following region: GGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CENPB (ARP51969_P050) antibody is Catalog # AAP51969 (Previous Catalog # AAPP40290)
• Reference: Orthaus,S., (2008) Chembiochem 9 (1), 77-92

Target Info

• Gene Symbol: CENPB
• Gene Full Name: Centromere protein B, 80kDa
• Alias Symbols: -
• NCBI Gene Id: 1059
• Protein Name: Major centromere autoantigen B
• Description of Target: CENPB is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P07199
• Protein Accession #: NP_001801
• Nucleotide Accession #: NM_001810
• Protein Size (# AA): 599
• Molecular Weight: 65kDa
• Protein Interactions: UBC; RNF2; NR3C1; FBXO25; CENPA; APP; GFI1B; SUMO2; SMN1; PIN1; GSK3B; GADD45A; CSNK2B; CDKN1A; ANXA7; RPS6KA6; TK1; TAC1; TAF1D; PARP1; PARP2; HAND2; CENPB;