Catalog No: OKCD00504 (Formerly GWB-KBBTK5)
Size:96WELLS
Price: $675.00
SKU
OKCD00504
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Datasheets/ManualsPrintable datasheet for CELSR2 ELISA Kit (Human) (OKCD00504)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CELSR2 Microplate96 Wells (12 x 8 Well Strips)
CELSR2 Lyophilized Standard2 x 10 ng
100X Biotinylated CELSR2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2). No significant cross-reactivity or interference between Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCELSR2
Gene Full Namecadherin EGF LAG seven-pass G-type receptor 2
Alias SymbolsADGRC2;adhesion G protein-coupled receptor C2;cadherin EGF LAG seven-pass G-type receptor 2;cadherin family member 10;cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila);CDHF10;EGFL2;EGF-like protein 2;EGF-like-domain, multiple 2;epidermal growth factor-like 2;epidermal growth factor-like protein 2;flamingo homolog 3;Flamingo1;MEGF3;multiple EGF-like domains protein 3;multiple epidermal growth factor-like domains 3;multiple epidermal growth factor-like domains protein 3.
NCBI Gene Id1952
Protein NameCadherin EGF LAG seven-pass G-type receptor 2
Description of TargetReceptor that may have an important role in cell/cell signaling during nervous system formation.
Uniprot IDQ9HCU4
Protein Accession #NP_001399
Nucleotide Accession #NM_001408.2
Protein Size (# AA)2923
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