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Catalog No: OKEH00248
Size:96T
Price: $525.00
SKU
OKEH00248
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CEACAM5 ELISA Kit (Human) : 96 Wells (OKEH00248)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CEACAM5 ELISA Kit (Human) (OKEH00248) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEACAM5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for CEACAM5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEACAM5 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
CEACAM5 Microplate96 Wells (12 x 8 Well strips)
CEACAM5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CEACAM5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.32 ng/mL
SpecificityNatural and recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCEACAM5
Gene Full NameCEA cell adhesion molecule 5
Alias SymbolsCarcinoembryonic antigen;carcinoembryonic antigen related cell adhesion molecule 5;carcinoembryonic antigen-related cell adhesion molecule 5;CD66e;CEA;meconium antigen 100.
NCBI Gene Id1048
Protein NameP06731
Description of TargetCarcinoembryonic antigen-related cell adhesion molecule 5
Uniprot IDhttps://www.uniprot.org/uniprot/P06731
Protein Accession #NP_001278413.1
Nucleotide Accession #NM_001291484.2
Protein Size (# AA)702
Molecular Weight77 kDa
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