CEACAM1 ELISA Kit (Human) (OKBB00622)

Catalog No: OKBB00622

Size:96 Wells

Human CEACAM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CEACAM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEACAM1 has been precoated onto 96-well plates. Standards(NS0, Q35-G428) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEACAM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CEACAM1 amount of sample captured in plate.
• ELISA Kit Range: 312 pg/ml - 20,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	5.14	7.35	12.1	5.36	8.04	13.5
  SD	0.185	0.397	0.593	0.295	0.571	0.837
  CV%	3.6	5.4	4.9	5.5	7.1	6.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human CEACAM1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human CEACAM1 Standard: 2 x 20 ng/tube
  • 100X Biotinylated Anti-Human CEACAM1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CEACAM1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q35-G428
• Description: For quantitative detection of human CEACAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CEACAM1
• Gene Full Name: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
• Alias Symbols: Antigen CD66, BGP, BGP 1, BGPI, Biliary glycoprotein, Biliary glycoprotein 1, Biliary glycoprotein adhesion molecule, Carcinoembryonic antigen related cell adhesion molecule 1, CD66a, CEA related cell adhesion molecule, CEACAM 1, CEACAM1
• NCBI Gene Id: 634
• Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 1
• Description of Target: CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.
• Uniprot ID: P13688
• Protein Accession #: NP_001020083.1
• Nucleotide Accession #: NM_001024912.2