CDKN2A Antibody (OAPB00526)

Catalog No: OAPB00526

Size:100UG

Western blot analysis of CDKN2A in mouse colon tissue lysate with CDKN2A antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of CDKN2A in human colon tissue with CDKN2A antibody at 10 ug/mL.

Immunohistochemistry of CDKN2A in rat colon tissue with CDKN2A antibody at 5 ug/ml.

Immunofluorescence of CDKN2A in Human Colon tissue with CDKN2A antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Mouse Colon Tissue Lysate (OCPB00076), Human Colon Tissue Lysate
• Reconstitution and Storage: CDKN2A antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-CDKN2A antibody (4211) was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CDKN2A. The immunogen is located within the first 50 amino acids of CDKN2A.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-CDKN2A (OAPB00526) antibody is Catalog # OEPB02645
• Intended Use: For Research Use Only
• Application Info: CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
• Reference: Stone S, Jiang P, Dayananth P, et al. Complex structure and regulation of the p16(MTS1) locus. Cancer Res.1995; 55:2988-94.;Kamb A, Shattuck-Eidens D, Eeles R, et al. Analysis of the p16 gene (CDKN2) as a candidate for the chromosome 9p melanoma susceptibility locus. Nature Genet.1994; 8:22-6.;Kratzke RA, Otterson GA, Lincoln CE, et al. Immunohistochemical analysis of the p16(INK4) cyclin-dependent kinase inhibitor in malignant mesothelioma. J. Nat. Cancer Inst.1995; 87:1870-5.;Stott FJ,; Bates S, James MC, et al. The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2. EMBO J.1998; 17:5001-14.

Target Info

• Gene Symbol: CDKN2A
• Gene Full Name: cyclin dependent kinase inhibitor 2A
• Alias Symbols: alternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16.
• NCBI Gene Id: 1029
• Protein Name: Cyclin-dependent kinase inhibitor 2A|cyclin-dependent kinase inhibitor 2A isoform p16INK4a [Homo sapiens]
• Description of Target: Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
• Uniprot ID: P42771
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000068
• Molecular Weight: Predicted: 17 kDa Observed: 17 kDa