Catalog No: OKEH04799
Size:96T
Price: $625.00
SKU
OKEH04799
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cdk5r1 ELISA Kit (Rat) (OKEH04799) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk5r1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk5r1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk5r1 captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Cdk5r1 Microplate96 Wells (12 x 8 Well Strips)
Cdk5r1 Standard2
Sample Diluent1 x 20 mL
Cdk5r1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.093 ng/mL
SpecificityNatural and recombinant Rat Cyclin-dependent kinase 5 activator 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCdk5r1
Gene Full Namecyclin-dependent kinase 5 regulatory subunit 1
Alias SymbolsCDK5 activator 1;Cdk5r;cyclin-dependent kinase 5 activator 1;Cyclin-dependent kinase 5 regulatory subunit 1;p23;p35;tau protein kinase II 23 kDa subunit;TPKII regulatory subunit.
NCBI Gene Id116671
Protein NameP61810
Description of TargetCyclin-dependent kinase 5 activator 1
Uniprot IDhttps://www.uniprot.org/uniprot/P61810
Protein Accession #NP_446343.1
Nucleotide Accession #NM_053891.1
Protein Size (# AA)307
Molecular Weight34 kDa
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