CDK5 Antibody (OAAB21019)

Catalog No: OAAB21019

Size:200ul

All lanes: Anti-CDK5 (OAAB21019) Antibody at 1:2,000 dilution Lane 1:A431 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

OAAB21019 staining CDK5 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody

Overlay histogram showing K562 cells stained with OAAB21019 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (OAAB21019, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (NA168821)) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling Pdx1 with OAAB21019 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide
• Clonality: Monoclonal
• Clone: 1552CT262.105.8
• Isotype: IgG1, kappa
• Host: Mouse
• Application: WB, IF, IHC-P, FC, ELISA
• Additional Information: Tissue Location: [Isoform 1]: Ubiquitously expressed. Accumulates in cortical neurons (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CDK5 antibody is generated from a mouse immunized with a recombinant protein of human CDK5.
• Purification: Protein G column purification, followed by dialysis against PBS
• Concentration: 0.5 mg/ml
• Application Info: IF: 1:25
  WB: 1:2000
  IHC-P: 1:25
  FC: 1:25
• Reference: Meyerson M.,et al.EMBO J. 11:2909-2917(1992).
  Meyerson M.,et al.Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases.
  Li Q.,et al.Mol. Biol. Rep. 37:2415-2421(2010).
  Hu X.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
  Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: CDK5
• Gene Full Name: cyclin-dependent kinase 5
• Alias Symbols: LIS7, PSSALRE
• NCBI Gene Id: 1020
• Description of Target: This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q00535
• Protein Accession #: NP_001157882.1
• Nucleotide Accession #: NM_001164410.2
• Molecular Weight: 33 kDa