CDK4 Antibody (OAAD00135)

Catalog No: OAAD00135

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: IML-4
• Isotype: IgG2a
• Host: Mouse
• Application: WB, IHC, ICC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Recombinant human Cdk4 protein.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 0.5-1ug/ml with the appropriate system to detect Cdk4 in cells and tissues.
  Immunocytochemistry Suitable Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Demetrick, D. J.; Zhang, H.; Beach, D. H. : Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994.
  2. Modiano, J. F.; Mayor, J.; Ball, C.; Fuentes, M. K.; Linthicum, D. S. : CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.
  e for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.

Target Info

• Gene Symbol: CDK4
• Alias Symbols: CMM3, PSK-J3
• NCBI Gene Id: 1019
• Protein Name: Cyclin-dependent kinase 4
• Description of Target: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
• Uniprot ID: P11802