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Catalog No: OKCD02443
Size:96WELLS
Price: $675.00
SKU
OKCD02443
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Datasheets/ManualsPrintable datasheet for CDK16 ELISA Kit (Human) (OKCD02443)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 16 (CDK16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 16 (CDK16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 16 (CDK16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cyclin Dependent Kinase 16 (CDK16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.25-2000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase 16 (CDK16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase 16 (CDK16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CDK16 Microplate96 Wells (12 x 8 Well strips)
CDK16 Lyophilized Standard2
100X Biotinylated CDK16 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity12.6 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 16 (CDK16). No significant cross-reactivity or interference between Cyclin Dependent Kinase 16 (CDK16) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCDK16
Gene Full Namecyclin dependent kinase 16
Alias Symbolscell division protein kinase 16;cyclin-dependent kinase 16;PCTAIRE;PCTAIRE1;PCTAIRE-motif protein kinase 1;PCTGAIRE;PCTK1;serine/threonine-protein kinase PCTAIRE-1;testis secretory sperm-binding protein Li 224n.
NCBI Gene Id5127
Protein NameCyclin-dependent kinase 16
Description of TargetProtein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Uniprot IDQ00536
Protein Accession #NP_001163931.1
Nucleotide Accession #NM_001170460.1
Protein Size (# AA)496
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