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Datasheets/Manuals | Printable datasheet for CDH5 Antibody (Phospho-Tyr731) (OAAF00512) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human VE-Cadherin around the phosphorylation site of Tyr731. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | VE-Cadherin (Phospho-Tyr731) antibody detects endogenous levels of VE-Cadherin only when phosphorylated at tyrosine 731. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000 |
Reference | Breviario F., Arterioscler. Thromb. Vasc. Biol. 15:1229-1239(1995). Ali J., Microcirculation 4:267-277(1997). Shimoyama Y., Biochem. J. 349:159-167(2000). |
Gene Symbol | CDH5 |
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Gene Full Name | cadherin 5 |
Alias Symbols | 7B4;7B4 antigen;cadherin 5, type 2 (vascular endothelium);cadherin 5, type 2, VE-cadherin (vascular epithelium);cadherin-5;CD144;cd144 antigen;endothelial-specific cadherin;vascular endothelial cadherin;VE-cadherin. |
NCBI Gene Id | 1003 |
Protein Name | Cadherin-5 |
Description of Target | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. |
Uniprot ID | P33151 |
Protein Accession # | NP_001786.2 |
Molecular Weight | 87 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
This target may also be called "7B4;7B4 antigen;cadherin 5, type 2 (vascular endothelium);cadherin 5, type 2, VE-cadherin (vascular epithelium);cadherin-5;CD144;cd144 antigen;endothelial-specific cadherin;vascular endothelial cadherin;VE-cadherin." in publications.
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What is the shipping cost for "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "87 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CDH5 Antibody (Phospho-Tyr731) (OAAF00512)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CDH5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CDH5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CDH5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CDH5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CDH5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CDH5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.