CDH17 ELISA Kit (Human) (OKCD00757)

Catalog No: OKCD00757 (Formerly GWB-KBBKG5)

Size:96WELLS

Human CDH17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-97%	82-104%	79-95%	94-102%
  EDTA plasma(n=5)	83-91%	96-104%	78-99%	85-92%
  heparin plasma(n=5)	82-93%	81-98%	88-96%	78-88%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 17 (CDH17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 17 (CDH17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 17 (CDH17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin 17 (CDH17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-97	94
  EDTA plasma(n=5)	80-102	88
  heparin plasma(n=5)	96-103	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin 17 (CDH17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin 17 (CDH17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CDH17 Microplate: 96 Wells (12 x 8 Well Strips)
  • CDH17 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated CDH17 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: < 11.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cadherin 17 (CDH17). No significant cross-reactivity or interference between Cadherin 17 (CDH17) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CDH17
• Gene Full Name: cadherin 17
• Alias Symbols: cadherin 17, LI cadherin (liver-intestine);cadherin-16;cadherin-17;CDH16;HPT1;HPT-1;HPT-1 cadherin;human intestinal peptide-associated transporter HPT-1;human peptide transporter 1;intestinal peptide-associated transporter HPT-1;LI cadherin;liver-intestine cadherin.
• NCBI Gene Id: 1015
• Protein Name: Cadherin-17
• Description of Target: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
• Uniprot ID: Q12864
• Protein Accession #: NP_001138135
• Nucleotide Accession #: NM_001144663.1
• Protein Size (# AA): 832