CD95 Antibody (OAAD00112)

Catalog No: OAAD00112

Size:0.5 ml

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
• Clonality: Monoclonal
• Clone: B500 (B-R18)
• Isotype: IgG1
• Host: Mouse
• Application: IHC, FC
• Additional Information: Recommended Positive Control: Tonsil, Spleen, Thymus
• Reconstitution and Storage: Store at 2° to 8°C for short term, freeze under -20°C for long term storage.
  Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies.
  Note: When diluting for Immunohistochemistry, ELISA or Western Blot, make the dilution in Antibody Diluting Buffer. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
• Immunogen: Purified recombinant Fas antigen
• Concentration: 0.3 mg/ml
• Specificity: This antibody reacts with CD95, a 40-45kD cell surface glycoprotein containing 8kD of N-glycosidic-linked polysaccharide and that is expressed by the CD45RAlow and CD45ROhigh subset of memory T cells.
• Application Info: IHC: 1:50 - 1:100
  FC: 1:150 - 1:300
  Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using with formalin-fixed paraffin embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC.
  Optimal dilutions should be determined by the end user.
• Reference: 1.) Trauth, B.C. etal, Science 245:301, 1989.
  2.) Debatin, K.M. etal, Blood 81:2972, 1993.
  3.) Moller, P. etal, Blood 81:2067, 1993.
  4.) Oehm, A. etal, J. Biol. Chem. 267:10709, 1992.
  5.) Itoh, N. etal, Cell 66:233, 1991.

Target Info

• Gene Symbol: FAS
• Alias Symbols: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6
• NCBI Gene Id: 355
• Protein Name: Tumor necrosis factor receptor superfamily member 6
• Description of Target: Monoclonal Mouse Anti-Human CD95 (FAS Antigen).
• Uniprot ID: P25445