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Catalog No: OKCD01128 (Formerly GWB-KBBES4)
Size:96WELLS
Price: $650.00
SKU
OKCD01128
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Datasheets/ManualsPrintable datasheet for CD83 ELISA Kit (Human) (OKCD01128)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-89%86-101%82-89%97-105%
EDTA plasma(n=5)78-91%92-99%98-105%96-104%
heparin plasma(n=5)89-102%89-103%99-105%83-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 83 (CD83). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 83 (CD83). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 83 (CD83), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 83 (CD83) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-9990
EDTA plasma(n=5)91-9996
heparin plasma(n=5)79-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 83 (CD83) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 83 (CD83) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CD83 Microplate96 Wells (12 x 8 Well Strips)
CD83 Lyophilized Standard2 x 200 pg
100X Biotinylated CD83 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, cell lysates and other biological fluids.
Sensitivity< 1.42 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 83 (CD83). No significant cross-reactivity or interference between Cluster Of Differentiation 83 (CD83) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCD83
Gene Full NameCD83 molecule
Alias SymbolsB-cell activation protein;BL11;CD83 antigen;CD83 antigen (activated B lymphocytes, immunoglobulin superfamily);cell surface protein HB15;cell-surface glycoprotein;HB15;hCD83.
NCBI Gene Id9308
Protein NameCD83 antigen
Description of TargetMay play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Uniprot IDQ01151
Protein Accession #NP_033986
Nucleotide Accession #NM_009856.3
Protein Size (# AA)205
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