Catalog No: OKCD00273 (Formerly GWB-KBBJB2)
Size:96WELLS
Price: $650.00
SKU
OKCD00273
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Datasheets/ManualsPrintable datasheet for CD72 ELISA Kit (Human) (OKCD00273)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 72 (CD72). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 72 (CD72). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 72 (CD72), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 72 (CD72) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 72 (CD72) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 72 (CD72) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CD72 Microplate96 Wells (12 x 8 Well Strips)
CD72 Lyophilized Standard2 x 10 ng
100X Biotinylated CD72 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 72 (CD72). No significant cross-reactivity or interference between Cluster Of Differentiation 72 (CD72) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCD72
Gene Full NameCD72 molecule
Alias SymbolsB-cell differentiation antigen CD72;CD72 antigen;CD72b;lyb-2;LYB2.
NCBI Gene Id971
Protein NameB-cell differentiation antigen CD72
Description of TargetPlays a role in B-cell proliferation and differentiation.
Uniprot IDP21854
Protein Accession #NP_001773
Nucleotide Accession #NM_001782.2
Protein Size (# AA)359
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