CD69 Antibody (OAEE00956)

Catalog No: OAEE00956

Size:100T

Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57). 

Product Info

• Predicted Species Reactivity: Human
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: FN50
• Isotype: IgG1
• Host: Mouse
• Conjugation: PerCP
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: anti-µ-stimulated human B lymphocytes
• Purification: Purified by size-exclusion chromatography
• Concentration: 10 ul / test
• Specificity: The antibody FN50 recognizes CD69, an lymphocyte early activation marker.
  HLDA IV; WS Code A 91
• Formulation: PerCP
• Storage Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
• Reference: Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286(22):19617-29.
  Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement forIFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104.
  Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989); p. 293.
  Konjević G, Jović V, Vuletić A, Radulović S, Jelić S, Spuzić I: CD69 on CD56+ NK cells and response to chemoimmunotherapy in metastatic melanoma. Eur J Clin Invest. 2007 Nov;37(11):887-96.
  Pitsios C, Dimitrakopoulou A, Tsalimalma K, Kordossis T, Choremi-Papadopoulou H: Expression of CD69 on T-cell subsets in HIV-1 disease. Scand J Clin Lab Invest. 2008;68(3):233-41.
  Nielsen SD, Afzelius P, Ersbøll AK, Nielsen JO, Hansen JE: Expression of the activation antigen CD69 predicts functionality of in vitro expanded peripheral blood mononuclear cells (PBMC) from healthy donors and HIV-infected patients. Clin Exp Immunol. 1998 Oct;114(1):66-72.
  López-Cabrera M, Santis AG, Fernández-Ruiz E, Blacher R, Esch F, Sánchez-Mateos P, Sánchez-Madrid F: Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors. J Exp Med. 1993 Aug 1;178(2):537-47.

Target Info

• Gene Symbol: CD69
• Gene Full Name: CD69 molecule
• Alias Symbols: Activation inducer molecule;activation inducer molecule (AIM/CD69);AIM;BL-AC/P26;CD69 antigen (p60, early T-cell activation antigen);CLEC2C;C-type lectin domain family 2 member C;C-type lectin domain family 2, member C;EA1;early activation antigen CD69;early lymphocyte activation antigen;early T-cell activation antigen p60;GP32/28;leukocyte surface antigen Leu-23;MLR-3.
• NCBI Gene Id: 969
• Protein Name: Early activation antigen CD69
• Description of Target: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
• Uniprot ID: Q07108
• Protein Accession #: NP_001772.1
• Nucleotide Accession #: NM_001781.2