CD6 ELISA Kit (Mouse) (OKBB00581)

Catalog No: OKBB00581

Size:96 Wells

Mouse CD6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD6 has been precoated onto 96-well plates. Standards(NS0, L18-G396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD6 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	798	1880	3505	1036	2040	3487
  SD	33.5	67.68	189.27	60	95.88	240.6
  CV%	4.2	3.6	5.4	5.8	4.7	6.9

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CD6 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CD6 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse CD6 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse CD6
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L18-G396
• Description: For quantitative detection of mouse CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CD6
• Gene Full Name: CD6 molecule
• Alias Symbols: CD_antigen=CD6, CD6, CD6 antigen Tp120, CD6 molecule, CD6_HUMAN, FLJ44171, OX52, T cell differentiation antigen CD6, T-cell differentiation antigen CD6, T12, TP120
• NCBI Gene Id: 12511
• Protein Name: T-cell differentiation antigen CD6
• Description of Target: CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.
• Uniprot ID: Q61003
• Protein Accession #: NP_001032890.1
• Nucleotide Accession #: NM_001037801.2