Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH03916
Size:96T
Price: $625.00
SKU
OKEH03916
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD46 ELISA Kit (Bovine) (OKEH03916) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD46 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD46 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD46 captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit Component
ComponentAmount
CD46 Microplate96 Wells (12 x 8 Well strips)
CD46 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CD46 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity12.3 pg/mL
SpecificityNatural and recombinant Bovine Membrane cofactor protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD46
Gene Full NameCD46 molecule
Alias Symbolsbrain microvascular membrane cofactor;CD46 molecule, complement regulatory protein;complement regulatory protein;MCP;membrane cofactor protein;membrane cofactor protein variant 10;membrane cofactor protein variant 7;membrane cofactor protein variant 8;membrane cofactor protein variant 9;soluble membrane cofactor protein variant 11;soluble membrane cofactor protein variant 12;soluble membrane cofactor protein variant 13;trophoblast-lymphocyte cross-reactive antigen.
NCBI Gene Id280851
Protein NameQ6VE48
Description of TargetMembrane cofactor protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q6VE48
Protein Accession #NP_001229490.1
Nucleotide Accession #NM_001242561.1
Protein Size (# AA)361
Molecular Weight361 kDa
Write Your Own Review
You're reviewing:CD46 ELISA Kit (Bovine) (OKEH03916)
Your Rating
We found other products you might like!