Catalog No: OKCD00171 (Formerly GWB-KBBYE6)
Size:96WELLS
Price: $700.00
SKU
OKCD00171
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Datasheets/ManualsPrintable datasheet for CD40 ELISA Kit (Rat) (OKCD00171)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-96%93-105%79-104%96-104%
EDTA plasma(n=5)82-93%83-92%98-105%99-105%
heparin plasma(n=5)94-105%97-105%91-98%78-90%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)91-9996
EDTA plasma(n=5)93-10198
heparin plasma(n=5)79-9086
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cd40 Microplate96 Wells (12 x 8 Well Strips)
Cd40 Lyophilized Standard2 x 1 ng
100X Biotinylated Cd40 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 5.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCd40
Gene Full NameCD40 molecule
Alias SymbolsCD40 antigen, TNF receptor superfamily member 5;CD40 molecule, TNF receptor superfamily member 5;Tnfrsf5;tumor necrosis factor receptor superfamily member 5;tumor necrosis factor receptor superfamily, member 5.
NCBI Gene Id171369
Protein NameCD40 molecule, TNF receptor superfamily member 5
Description of TargetCD40 molecule, TNF receptor superfamily member 5
Uniprot IDQ4QQW2
Protein Accession #NP_599187
Nucleotide Accession #NM_134360.1
Protein Size (# AA)289
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