CD4 Antibody (OASA00630)

Catalog No: OASA00630 (Formerly GWB-Q00926)

Size:2ML

Product Info

• Predicted Species Reactivity: Dog
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: CA13.1E4
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Frozen|Immunoprecipitation
• Additional Information: Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 mouse myeloma cell line.
  Histology Positive Control Tissue: Spleen, lymph node
• Reconstitution and Storage: -20°C
• Immunogen: Canine Thymocytes
• Predicted Homology Based on Immunogen Sequence: Dog
• Preservative: 0.1% - Sodium Azide
• Specificity: Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobbold et al.1992 ] and identifies, by immunoprecipitation a 60kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper
  cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Moore et al. 1992).
  CD4 expression has also been reported on a population of CD3 positive peripheral blood T
  lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar
  reports have been made for cynomolgus monkey (Nam et al. 2000), pig (Saalmuller et al. 1987
  , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).
  Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on
  canine neutrophils at a similar density to that expressed on canine T helper cells. The functional
  significance of this is not understood and remains enigmatic in view of the roles described for
  CD4 in canine and other mammalian species (Moore et al. 1992).
  Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone
  macrophages (Moore et al. 1992). Other macrophage populations, such as splenic red pulp
  macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T
  cells can be further characterized according to their simultaneous expression of CD45RA as
  recognized by Rat anti Canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen
  with human T cells (Moore et al. 1992).
  Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for
  the study of various lymphoploriferative diseases. T lymphocytes in large granular
  lymophocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs
  with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrant et
  al. 2001). CD4 expression varies considerably between various canine leukemias (Villiers 2002
  ).
  
• Application Info: Flow Cytometry: Neat
  Use 25ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.
• Reference: 1. Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils. Tissue Antigens 40(2): 75-85.
  2. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the first International Canine Leucocyte Antigen Workshop (CLAW). Tissue Antigens 43(3): 137-154.
  3. Veenhof, E.Z. et al. (2010) Characterisation of T cell phenotypes, cytokines and transcription factors in the

Target Info

• Gene Symbol: CD4
• NCBI Gene Id: 403931
• Protein Name: T-cell surface glycoprotein CD4
• Description of Target: MOUSE ANTI DOG CD4
  
• Uniprot ID: P33705
• Protein Accession #: NP_001003252.1
• Protein Size (# AA): 463