CD4 Antibody (OAEE00852)

Catalog No: OAEE00852

Size:0.1 mg

Product Info

• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: GK1.5
• Isotype: IgG2b
• Host: Rat
• Application: FC, IP, IHC-F, ICC, FN
• Reconstitution and Storage: Store in the dark at 2-8C. Do not freeze. Avoid prolonged exposure to light.
  Do not use after expiration date stamped on vial label.
  Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
• Immunogen: Mouse CTL clone V4 cells
• Purification: Purified by size-exclusion chromatography
• Concentration: 0.5 mg/ml
• Specificity: The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
• Formulation: PerCP
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85(8):1167-74.
  Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J Immunol. 2008 May 15;180(10):6593-603.
  Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes. Nat Immunol. 2007 Apr;8(4):388-97.
  Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996 Sep 1;184(3):1083-91.
  Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia. Am J Respir Cell Mol Biol. 1994 Jun;10(6):587-93.
  Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL-12 influences intrathymic T cell development. J Immunol. 1994 Mar 15;152(6):2729-35.
  Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991 Dec 1;174(6):1617-27.
  Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibodyGK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.
  Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.
  Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al.: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29-56.
  

Target Info

• Gene Symbol: CD4
• Gene Full Name: T-cell surface glycoprotein CD4
• Alias Symbols: IMD79, OKT4D, CD4mut
• NCBI Gene Id: 920
• Protein Name: T-cell surface glycoprotein CD4
• Uniprot ID: P01730
• Protein Accession #: NP_000607.1
• Nucleotide Accession #: NM_000616.4