CD4 Antibody (OAEE00228)

Catalog No: OAEE00228

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-16
• Isotype: IgM
• Host: Mouse
• Application: Flow cytometry|Immunoprecipitation
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: HPB cell line (human peripheral blood leukemia T-cells)
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T-cells) and also on monocytes, tissue macrophages and granulocytes.

  
  HLDA V; WS Code T T-CD04.10
  HLDA VI; WS Code T 6T-013

• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
• Reference: Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing, Inc., New York (1998); p. 49-54.
  Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53(1):33-40.
  Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2008-13., Clapham PR, McKnight A.: Cell surface receptors, virus entry and tropism of primate lentiviruses. J Gen Virol. 2002 Aug;83(Pt 8):1809-29.

Target Info

• Gene Symbol: CD4
• Gene Full Name: CD4 molecule
• Alias Symbols: CD4 antigen (p55);CD4 receptor;CD4mut;IMD79;OKT4D;T-cell surface antigen T4/Leu-3;T-cell surface glycoprotein CD4.
• NCBI Gene Id: 920
• Protein Name: T-cell surface glycoprotein CD4
• Description of Target: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
• Uniprot ID: P01730
• Protein Accession #: NP_000607.1
• Nucleotide Accession #: NM_000616.4