CD4 Antibody (OAEE00224)

Catalog No: OAEE00224

Size:100UG

Immunoprecipitation of human CD4 from
the lysate T cells isolated from fresh buffy coats.
Western blot was immunostained by anti-human
CD4 (MEM-241).
Lane 1: original lysate of T cells
Lane 2: immunoprecipitate by negative control antibody
Lane 3: immunoprecipitate by anti-human CD4 (MEM-115)

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4
• Clonality: Monoclonal
• Clone: MEM-115
• Isotype: IgG2a
• Host: Mouse
• Application: Flow cytometry|Functional assay|Immunoprecipitation
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Human thymocytes and T lymphocytes.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
  HLDA V; WS Code T T-CD04.09
• Formulation: Azide free
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.
• Reference: Singer II, Scott S, Kawka DW, Chin J, Daugherty BL, DeMartino JA, DiSalvo J, Gould SL, Lineberger JE, Malkowitz L, Miller MD, Mitnaul L, Siciliano SJ, Staruch MJ, Williams HR, Zweerink HJ, Springer MS: CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of humanmacrophages and T cells.
  Bosze S, Caccamo N, Majer Z, Mezo G, Dieli F, Hudecz F: In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis. Biopolymers. 2004;76(6):467-76.
  Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53(1):33-40. , Clapham PR, McKnight A.: Cell surface receptors, virus entry and tropism of primate lentiviruses. J Gen Virol. 2002 Aug;83(Pt 8):1809-29.
  Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2008-13.

Target Info

• Gene Symbol: CD4
• Gene Full Name: CD4 molecule
• Alias Symbols: CD4 antigen (p55);CD4 receptor;CD4mut;IMD79;OKT4D;T-cell surface antigen T4/Leu-3;T-cell surface glycoprotein CD4.
• NCBI Gene Id: 920
• Protein Name: T-cell surface glycoprotein CD4
• Description of Target: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
• Uniprot ID: P01730
• Protein Accession #: NP_000607.1
• Nucleotide Accession #: NM_000616.4