CD4 Antibody (OAAF00988)

Catalog No: OAAF00988 (Formerly GWB-ASC395)

Size:100 ug

Western blot analysis of extracts from COLO205 cells, using CD4 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CD4 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using CD4 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CD4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: CD4 (Ab-433) antibody detects endogenous levels of total CD4 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Reference: GR Harriman, J. Immunol., Oct 1990; 145: 2406.
  G Zauli, Blood, Sep 1994; 84: 1896 - 1905.
  Andrew Zloza, Blood, Sep 2003; 102: 2156 - 2164.

Target Info

• Gene Symbol: CD4
• Gene Full Name: CD4 molecule
• Alias Symbols: CD4 antigen (p55);CD4 receptor;CD4mut;IMD79;OKT4D;T-cell surface antigen T4/Leu-3;T-cell surface glycoprotein CD4.
• NCBI Gene Id: 920
• Protein Name: T-cell surface glycoprotein CD4
• Description of Target: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
• Uniprot ID: P01730
• Protein Accession #: NP_000607.1
• Molecular Weight: 51 kDa