Catalog No: OKCD00672 (Formerly GWB-KBBVT7)
Size:96WELLS
Price: $650.00
SKU
OKCD00672
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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Datasheets/Manuals | Printable datasheet for CD300LF ELISA Kit (Mouse) (OKCD00672) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immune Receptor Expressed On Myeloid Cells 1 (IREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Immune Receptor Expressed On Myeloid Cells 1 (IREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immune Receptor Expressed On Myeloid Cells 1 (IREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immune Receptor Expressed On Myeloid Cells 1 (IREM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.62 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Immune Receptor Expressed On Myeloid Cells 1 (IREM1). No significant cross-reactivity or interference between Immune Receptor Expressed On Myeloid Cells 1 (IREM1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Cd300lf |
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Gene Full Name | CD300 molecule like family member F |
Alias Symbols | CD300 antigen like family member F;CD300 antigen-like family member F;CLIM1;CLM1;CLM-1;CMRF35-like molecule 1;CMRF-35-like molecule 1;DC-derived Ig-like receptor 2;DIg;Digr2;F730004D16Rik;IgSF;IgSF13;IRE;IREM1;leukocyte mono-Ig-like receptor 3;LM;LMIR3;MAIR-5;MAIR-V;myeloid-associated immunoglobulin-like receptor 5;Pi;Pigr3. |
NCBI Gene Id | 246746 |
Protein Name | CMRF35-like molecule 1 |
Description of Target | Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:17438331). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells (PubMed:21865548). Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (PubMed:26768664). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand (PubMed:23123064). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (PubMed:26124135). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (By similarity). Inhibits osteoclast formation (PubMed:14662855). Induces macrophage cell death upon engagement (PubMed:18097021). |
Uniprot ID | Q6SJQ7 |
Protein Accession # | NP_001162624 |
Nucleotide Accession # | NM_001169153.1 |
Protein Size (# AA) | 337 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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