CD200 ELISA Kit (Human) : 96 Wells (OKEH02676)

Catalog No: OKEH02676

Size:96T

Human CD200 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CD200 ELISA Kit (Human) (OKEH02676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD200 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CD200 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD200 captured in well.
• ELISA Kit Range: 125-8000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.3% (n = 20)
  Mean Inter-assay CV%: < 9.5% (n = 20)
• ELISA Kit Component:
  • Anti-CD200 Microplate: 96 Wells (12 x 8 Well strips)
  • CD200 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CD200 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 56 pg/mL
• Specificity: Natural and recombinant Human OX-2 membrane glycoprotein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD200
• Gene Full Name: CD200 molecule
• Alias Symbols: antigen identified by monoclonal antibody MRC OX-2;CD200 antigen;MOX1;MOX2;MRC;OX-2;OX-2 membrane glycoprotein.
• NCBI Gene Id: 4345
• Protein Name: P41217
• Description of Target: OX-2 membrane glycoprotein
• Uniprot ID: https://www.uniprot.org/uniprot/P41217
• Protein Accession #: NP_001004196.2
• Nucleotide Accession #: NM_001004196.3
• Protein Size (# AA): 278
• Molecular Weight: 31 kDa