CD163 ELISA Kit (Mouse) (OKBB00515)

Catalog No: OKBB00515

Size:96 Wells

Mouse CD163 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD163 has been precoated onto 96-well plates. Standards(NS0, V39-T1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD163 amount of sample captured in plate.
• ELISA Kit Range: 0.78 ng/ml - 50 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	8.1	15.6	34	9.2	17.8	32
  SD	0.33	0.69	1.9	0.57	1.01	1.7
  CV%	4.1	4.4	5.6	6.2	5.7	5.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CD163 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CD163 Standard: 2 x 50 ng/tube
  • 100X Biotinylated Anti-Mouse CD163 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin)
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant mouse CD163
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: V39-T1045
• Description: For quantitative detection of mouse CD163 in cell culture supernatants, serum and plasma(heparin).

Target Info

• Gene Symbol: CD163
• Gene Full Name: CD163 molecule
• Alias Symbols: CD_antigen=CD163, CD163, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130
• NCBI Gene Id: 93671
• Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Description of Target: CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
• Uniprot ID: Q2VLH6
• Protein Accession #: NP_444324.2
• Nucleotide Accession #: NM_053094.2