CD142 ELISA Kit (Rabbit) (OKCD01132)

Catalog No: OKCD01132 (Formerly GWB-KBBPA2)

Size:96WELLS

Rabbit CD142 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-91%	87-101%	96-104%	82-102%
  EDTA plasma(n=5)	85-101%	89-101%	86-101%	78-90%
  heparin plasma(n=5)	87-96%	99-105%	85-99%	80-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Factor (TF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Factor (TF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Factor (TF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tissue Factor (TF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	89-96	92
  EDTA plasma(n=5)	80-90	87
  heparin plasma(n=5)	82-89	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CD142 Microplate: 96 Wells (12 x 8 Well Strips)
  • CD142 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated CD142 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: < 21.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tissue Factor (TF). No significant cross-reactivity or interference between Tissue Factor (TF) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: F3
• Gene Full Name: coagulation factor III, tissue factor
• Alias Symbols: brain tissue factor;Coagulation factor III;coagulation factor III (thromboplastin, tissue factor);TF;tissue factor.
• NCBI Gene Id: 100009127
• Protein Name: Tissue factor
• Description of Target: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
• Uniprot ID: P24055
• Protein Accession #: NP_001075760
• Nucleotide Accession #: NM_001082291.1
• Protein Size (# AA): 292