CCS ELISA Kit (Rat) (OKCD00247)

Catalog No: OKCD00247 (Formerly GWB-KBBXH4)

Size:96WELLS

Rat Ccs ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase Copper Chaperone. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase Copper Chaperone. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase Copper Chaperone, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Superoxide Dismutase Copper Chaperone in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase Copper Chaperone were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase Copper Chaperone were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ccs Microplate: 96 Wells (12 x 8 Well Strips)
  • Ccs Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Ccs Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase, Copper Chaperone (SOD4). No significant cross-reactivity or interference between Superoxide Dismutase, Copper Chaperone (SOD4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ccs
• Gene Full Name: copper chaperone for superoxide dismutase
• Alias Symbols: copper chaperone for superoxide dismutase;superoxide dismutase copper chaperone.
• NCBI Gene Id: 84485
• Protein Name: Copper chaperone for superoxide dismutase
• Description of Target: Delivers copper to copper zinc superoxide dismutase (SOD1).
• Uniprot ID: Q9JK72
• Protein Accession #: NP_445877
• Nucleotide Accession #: NM_053425.1
• Protein Size (# AA): 274