Catalog No: OKCD02434
Size:96WELLS
Price: $625.00
SKU
OKCD02434
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for CCR2 ELISA Kit (Mouse) (OKCD02434) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 2 (CCR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 2 (CCR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 2 (CCR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-C-Motif Receptor 2 (CCR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 78-5,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 2 (CCR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 2 (CCR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids | ||||||||||||||||||||||
Sensitivity | 34 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 2 (CCR2). No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 2 (CCR2) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | Ccr2 |
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Gene Full Name | chemokine (C-C motif) receptor 2 |
Alias Symbols | C-C chemokine receptor type 2;C-C CHEMOKINE RECEPTOR TYPE 2 (C-C CKR-2) (CC-CKR-2) (CCR-2) (CCR2) (JE/FIC RECEPTOR) (MCP-1 RECEPTOR);C-C CKR-2;CC-CKR-;Cc-ckr-2;CCR;CCR-2;Ccr2a;Ccr2b;chemoattractant protein-1 receptor;chemokine (C-C) receptor 2;CKR;Ckr2;Ckr2a;Ckr2b;Cmkbr;Cmkbr2;JE/FIC receptor;MCP-1 receptor;MIP-1 alphaR;mJe-r. |
NCBI Gene Id | 12772 |
Protein Name | C-C chemokine receptor type 2 |
Description of Target | Receptor for the CCL2, CCL7 and CCL12 chemokines (PubMed:8996246). Receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Transduces a signal by increasing intracellular calcium ion levels (PubMed:8996246). Upon CCL2 ligation, mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity). |
Uniprot ID | P51683 |
Protein Accession # | NP_034045.1 |
Nucleotide Accession # | NM_009915.2 |
Protein Size (# AA) | 373 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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