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CHN2(chimerin (chimaerin) 2) Antibody

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Catalog#   Accession # Size Price Qty
ARP51862_P050   NP_001035025 50µg $289.00
Description: 
CHN2 is a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. CHN2 plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men.This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol: 
CHN2  
Alias Symbols: 
ARHGAP3; BCH; MGC138360; RHOGAP3  
Peptide ID: 
P40041  
ELISA titre using peptide based assay: 
1:62500  
Search Keyword: 
CHN2, NM_001039936, NP_001035025, P52757  
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Species Reactivity: 
(Primary - WB; Secondary - Computational Homology)
Human, Mouse, Rat 
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CHN2 with an internal ID of P40041 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Uhl,G.R., (2008) Arch. Gen. Psychiatry 65 (6), 683-693 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 1¦Ìg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP51862-QC21775-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_001035025 P52757 NM_001039936 332 38kDa