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AARS(alanyl-tRNA synthetase) Antibody

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Catalog#   Accession # Size Price Qty
ARP40369_P050   NP_001596 50µg $289.00
Description: 
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Species Reactivity: 
Human,Mouse  
Gene Symbol: 
AARS  
Alias Symbols: 
-  
Peptide ID: 
P22113  
ELISA titre using peptide based assay: 
1:312500  
Search Keyword: 
AARS, NM_001605, NP_001596, P49588  
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Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AARS with an internal ID of P22113 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Olsen,J.V., (2006) Cell 127 (3), 635-648 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP40369-QC21124-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_001596 P49588 NM_001605 968 107kDa