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AASDHPPT(aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase) Antibody


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Catalog#   Accession # Size Price Qty
ARP48777_P050   NP_056238 50µg $289.00
Description: 
AASDHPPT is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia.The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia.
Gene Symbol: 
AASDHPPT  
Alias Symbols: 
AASD-PPT; CGI-80; DKFZp566E2346; LYS2; LYS5  
Peptide ID: 
P28828  
ELISA titre using peptide based assay: 
1:12500  
Search Keyword: 
AASDHPPT, NM_015423, NP_056238, Q9NRN7  
Click Here to view more information (protein size, molecular weight, etc)
Species Reactivity: 
(Primary - WB; Secondary - Computational Homology)
Human 
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AASDHPPT with an internal ID of P28828 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Joshi,A.K., (2003) J. Biol. Chem. 278 (35), 33142-33149 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP48777-QC20064-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_056238 Q9NRN7 NM_015423 309 36kDa