A4GNT(alpha-1,4-N-acetylglucosaminyltransferase) Antibody

Preview

Catalog#

Accession #

Size

Price

Qty

ARP47221_P050

NP_057245

50µg

$289.00

Description:
A4GNT is a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

Species Reactivity:
Human

Gene Symbol:
A4GNT

Alias Symbols:
MGC149493; alpha4GnT

Peptide ID:
P27991

ELISA titre using peptide based assay:
1:62500

Search Keyword:
A4GNT, NM_016161, NP_057245, Q9UNA3

Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human A4GNT with an internal ID of P27991

Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Key Reference:
Ishizone,S., (2006) Cancer Sci. 97 (2), 119-126

Quality Control:
Rabbit Ig G is purified by peptide affinity chromatography method.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot
Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Immunoblot

ARP47221-QC19167-WB.jpg

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_057245	Q9UNA3	NM_016161	340	39kDa