NR2F2(nuclear receptor subfamily 2, group F, member 2) Antibody

Preview

Catalog#

Accession #

Size

Price

Qty

ARP39465_T100

NP_066285

100µg

$229.00

Description:
NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.

Species Reactivity:
Human,Mouse,Rat,Dog

Gene Symbol:
NR2F2

Alias Symbols:
ARP1; COUP-TFII; COUPTFB; MGC117452; SVP40; TFCOUP2

Peptide ID:
P21479

ELISA titre using peptide based assay:
1:312500

Search Keyword:
NR2F2, NM_021005, NP_066285, P24468

Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human NR2F2 with an internal ID of P21479

Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Key Reference:
Sato,Y., (2003) J. Clin. Endocrinol. Metab. 88 (7), 3415-3420

Quality Control:
Rabbit IgG purified by protein A chromatography.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot
Suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Immunoblot

ARP39465-QC14702-WB.jpg

Immunohistochemistry 1

ARP39465-QC14702-IHC.jpg

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_066285	P24468	NM_021005	414	45kDa