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APOH(apolipoprotein H (beta-2-glycoprotein I)) Antibody

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Catalog#   Accession # Size Price Qty
ARP51158_P050   NP_000033 50µg $289.00
Description: 
Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.
Species Reactivity: 
Human,Dog  
Gene Symbol: 
APOH  
Alias Symbols: 
B2G1; BG  
Peptide ID: 
P28029  
ELISA titre using peptide based assay: 
1:312500  
Search Keyword: 
APOH, NM_000042, NP_000033, P02749  
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Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human APOH with an internal ID of P28029 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Fischetti,F., (2005) Blood 106 (7), 2340-2346 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP51158-QC16599-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_000033 P02749 NM_000042 345 36kDa