AGPAT2(1-acylglycerol-3-phosphate O-acyltransferase 2 ) Antibody

• Catalog#: ARP44637_T100
• Accession#: NP_001012745
• Size: 100µg

Description: 
AGPAT2 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in its gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance.This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Species Reactivity:  Human   Gene Symbol:  AGPAT2   Alias Symbols:  1-AGPAT2; BSCL; BSCL1; LPAAB; LPAAT-beta   Peptide ID:  P12109   ELISA titre using peptide based assay:  1:312500   Search Keyword:  AGPAT2, NM_001012727, NP_001012745, Q5VUD3, NM_006412, NP_006403, O15120   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AGPAT2 with an internal ID of P12109  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Niesporek,S., (2005) Br. J. Cancer 92 (9), 1729-1736  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP44637-QC14744-WB.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_001012745	Q5VUD3	NM_001012727	246	27kDa
NP_006403	O15120	NM_006412	278	31kDa