NR1I2(nuclear receptor subfamily 1, group I, member 2) Antibody

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Catalog#

Accession #

Size

Price

Qty

ARP39738_T100

NP_003880

100µg

$229.00

Description:
NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. NR1I2 contains a zinc finger domain.NR1I2 is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain.The gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. The gene product contains a zinc finger domain. Three alternatively spliced transcripts that encode different isoforms have been described, one of which encodes two products through the use of alternative translation initiation codons. Additional transcript variants derived from alternative promoter usage, alternative splicing, and/or alternative polyadenylation exist, but they have not been fully described.

Gene Symbol:
NR1I2

Alias Symbols:
BXR; ONR1; PAR; PAR1; PAR2; PARq; PRR; PXR; SAR; SXR

Peptide ID:
P21763

ELISA titre using peptide based assay:
1:312500

Search Keyword:
NR1I2, NM_003889, NP_003880, O75469, NM_033013, NP_148934, O75469

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Species Reactivity:
(Primary - WB; Secondary - Computational Homology)
Human

Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human NR1I2 with an internal ID of P21763

Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Key Reference:
Dring,M.M., (2006) Gastroenterology 130 (2), 341-348

Quality Control:
Rabbit IgG purified by protein A chromatography.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot
Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Immunoblot

ARP39738-QC14703-WB.jpg

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_003880	O75469	NM_003889	434	50kDa
NP_148934	O75469	NM_033013	397	46kDa